{"id":2183,"date":"2025-07-10T13:28:45","date_gmt":"2025-07-10T13:28:45","guid":{"rendered":"https:\/\/phoenixlab1.com\/?page_id=2183"},"modified":"2025-07-27T09:50:06","modified_gmt":"2025-07-27T09:50:06","slug":"modello-acuto-e-cronico-indotto-dal-dextran-sulfate-sodium-dss","status":"publish","type":"page","link":"https:\/\/phoenixlab1.com\/index.php\/modello-acuto-e-cronico-indotto-dal-dextran-sulfate-sodium-dss\/","title":{"rendered":"Modello acuto e cronico indotto dal Dextran sulfate sodium (DSS)"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-page\" data-elementor-id=\"2183\" class=\"elementor elementor-2183\" data-elementor-settings=\"{&quot;element_pack_global_tooltip_width&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;element_pack_global_tooltip_width_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;element_pack_global_tooltip_width_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;element_pack_global_tooltip_padding&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true},&quot;element_pack_global_tooltip_padding_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true},&quot;element_pack_global_tooltip_padding_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true},&quot;element_pack_global_tooltip_border_radius&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true},&quot;element_pack_global_tooltip_border_radius_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true},&quot;element_pack_global_tooltip_border_radius_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true}}\">\n\t\t\t\t<div class=\"elementor-element elementor-element-f941c6e e-con-full e-flex e-con e-parent\" data-id=\"f941c6e\" data-element_type=\"container\" data-e-type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;video&quot;,&quot;background_video_link&quot;:&quot;https:\\\/\\\/phoenixlab1.com\\\/wp-content\\\/uploads\\\/2025\\\/06\\\/Animation.mp4&quot;,&quot;background_video_start&quot;:0,&quot;background_play_on_mobile&quot;:&quot;yes&quot;}\">\n\t\t<div class=\"elementor-background-video-container\">\n\t\t\t\t\t\t\t<video class=\"elementor-background-video-hosted\" role=\"presentation\" autoplay muted playsinline loop><\/video>\n\t\t\t\t\t<\/div><div class=\"elementor-element elementor-element-f8d2276 e-flex e-con-boxed e-con e-child\" data-id=\"f8d2276\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-dcbee3b elementor-widget elementor-widget-heading\" data-id=\"dcbee3b\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Modello acuto e cronico indotto dal Dextran sulfate sodium (DSS)<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-a702c0b e-flex e-con-boxed e-con e-parent\" data-id=\"a702c0b\" data-element_type=\"container\" data-e-type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-3c62afa elementor-widget elementor-widget-spacer\" data-id=\"3c62afa\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-98adfc7 elementor-widget elementor-widget-text-editor\" data-id=\"98adfc7\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-weight: 400;\"><span style=\"color: #cfaf7a; font-weight: bold;\">\ud83d\udd0d\u00a0 PER<b>CH\u00c9 \u00c8 UTILE? <\/b><\/span>Il destrano solfato di sodio (DSS) \u00e8 ampiamente utilizzato per generare modelli murini di <span style=\"color: #cfaf7a; font-weight: bold;\">colite ulcerosa<\/span>\u00a0e la\u00a0<span style=\"color: #cfaf7a; font-weight: bold;\">colite ulcerosa<\/span>, grazie alla sua capacit\u00e0 di danneggiare selettivamente l\u2019epitelio intestinale e compromettere l\u2019integrit\u00e0 della barriera mucosale del colon distale e rettale. Questo danno induce manifestazioni cliniche coerenti con le patologie umane: <span style=\"color: #cfaf7a; font-weight: bold;\">calo ponderale, diarrea e sangue occulto nelle feci<\/span>, rendendolo uno strumento validato per studi traslazionali nelle IBD<b>. <span style=\"color: #cfaf7a; font-weight: bold;\">Viene utilizzato per testare l\u2019efficacia terapeutica di nuovi farmaci.<\/span><\/b><\/span><\/p><p><span style=\"font-weight: 400;\"><span style=\"color: #cfaf7a; font-weight: bold;\">\ud83d\udd0d\u00a0 COME FUNZIONA IL MODELLO?<b> <\/b><\/span>La <span style=\"color: #cfaf7a; font-weight: bold;\">fase acuta<\/span> <\/span><span style=\"font-size: 16px; font-weight: 400;\">\u00e8 caratterizzata da una marcata attivazione di <\/span><span style=\"color: #cfaf7a; font-weight: bold;\">colite neutrofili e macrofagi<\/span><span style=\"font-size: 16px; font-weight: 400;\">. Nelle <span style=\"font-weight: 400;\"><span style=\"color: #cfaf7a; font-weight: bold;\">fasi croniche<\/span><\/span>, invece, si osserva un <span style=\"font-weight: 400;\"><span style=\"color: #cfaf7a; font-weight: bold;\">maggiore coinvolgimento dei linfociti<\/span><\/span>, in linea con le dinamiche immunitarie che si riscontrano nei pazienti affetti da IBD. <span style=\"font-weight: 400;\"><span style=\"color: #cfaf7a; font-weight: bold;\">Il modello \u00e8 ben validato e garantisce un\u2019elevata riproducibilit\u00e0 sperimentale.<\/span><\/span><\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ed701af elementor-widget elementor-widget-image\" data-id=\"ed701af\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"1024\" height=\"369\" src=\"https:\/\/phoenixlab1.com\/wp-content\/uploads\/2025\/07\/cattura21-1024x369.png\" class=\"attachment-large size-large wp-image-2409\" alt=\"\" srcset=\"https:\/\/phoenixlab1.com\/wp-content\/uploads\/2025\/07\/cattura21-1024x369.png 1024w, https:\/\/phoenixlab1.com\/wp-content\/uploads\/2025\/07\/cattura21-300x108.png 300w, https:\/\/phoenixlab1.com\/wp-content\/uploads\/2025\/07\/cattura21-768x277.png 768w, https:\/\/phoenixlab1.com\/wp-content\/uploads\/2025\/07\/cattura21.png 1119w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-17e9f00 elementor-widget__width-initial elementor-widget elementor-widget-text-editor\" data-id=\"17e9f00\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><i><span style=\"font-weight: 400;\">L\u2019 immagine mostra l\u2019 andamento della fase acuta e cronica dell\u2019 infiammazione del colon indotta dal DSS.\u00a0<\/span><\/i><b><i><\/i><\/b><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-7597673 elementor-widget__width-initial elementor-widget elementor-widget-text-editor\" data-id=\"7597673\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-weight: 400;\">Nel modello di <span style=\"color: #cfaf7a; font-weight: bold;\">colite acuta <\/span><\/span><span style=\"font-weight: 400;\">indotta da DSS, topi C57\/Bl6 di 8-12 settimane vengono sottoposti a un ciclo orale (tramite acqua da bere) di DSS al 2,5-3% (massa molecolare 40 kDa), caratterizzato da 7 giorni di esposizione al DSS seguiti da 3-7 giorni di acqua depurata.<\/span><\/p><p><span style=\"font-weight: 400;\">Nel modello di <span style=\"color: #cfaf7a; font-weight: bold;\"><b>colite cronica<\/b><\/span><\/span><b>\u00a0<\/b><span style=\"font-weight: 400;\">i topi C57\/Bl6 di 8-12 settimane vengono sottoposti a 3 cicli orali di DSS al 2%, caratterizzato da 7 giorni di esposizione al DSS seguiti da 7 giorni di acqua depurata.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-54b23af elementor-widget__width-initial elementor-widget elementor-widget-text-editor\" data-id=\"54b23af\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-weight: 400;\"><span style=\"color: #cfaf7a; font-weight: bold;\"><b>IL MODELLO SIA ACUITO CHE CRONICO VIENE ESEGUITO CON SUCCESSO ANCHE NEI RATTI.<\/b><\/span><\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-befa974 elementor-widget elementor-widget-spacer\" data-id=\"befa974\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-39bef37 elementor-widget elementor-widget-heading\" data-id=\"39bef37\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">PARAMETRI VALUTATI<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-defcd24 elementor-widget elementor-widget-text-editor\" data-id=\"defcd24\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-weight: 400;\">1) Parametri clinici: <\/span><b>Perdita di peso corporeo <\/b><span style=\"font-weight: 400;\">e<\/span> <b>Disease Activity Index (DAI)<\/b><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-5c198ea elementor-widget elementor-widget-image\" data-id=\"5c198ea\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"1024\" height=\"282\" src=\"https:\/\/phoenixlab1.com\/wp-content\/uploads\/2025\/07\/Capture22-1024x282.png\" class=\"attachment-large size-large wp-image-2419\" alt=\"\" srcset=\"https:\/\/phoenixlab1.com\/wp-content\/uploads\/2025\/07\/Capture22-1024x282.png 1024w, https:\/\/phoenixlab1.com\/wp-content\/uploads\/2025\/07\/Capture22-300x83.png 300w, https:\/\/phoenixlab1.com\/wp-content\/uploads\/2025\/07\/Capture22-768x212.png 768w, https:\/\/phoenixlab1.com\/wp-content\/uploads\/2025\/07\/Capture22.png 1280w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-69bc1d1 elementor-widget elementor-widget-text-editor\" data-id=\"69bc1d1\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-weight: 400;\">2) Parametri endoscopici: <\/span><b>Traslucenza della mucosa, cambiamenti del pattern vascolare, depositi di fibrina, granulosit\u00e0 mucosale<\/b><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-cbe67ba elementor-widget__width-initial elementor-widget elementor-widget-text-editor\" data-id=\"cbe67ba\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-weight: 400;\">Eseguiamo regolarmente analisi endoscopiche in modelli murini di IBD.\u00a0<\/span><\/p><p><span style=\"font-weight: 400;\">L\u2019apparato sperimentale per l\u2019endoscopia, denominato \u201cColoview system\u201d, \u00e8 composto da un endoscopio in miniatura (diametro esterno di 1,9 mm), una fonte di luce allo xeno, una telecamera a triplo chip e una pompa d\u2019aria (Karl Storz, Tuttlingen, Germania).<\/span><\/p><p><span style=\"font-weight: 400;\">La procedura endoscopica viene visualizzata su un monitor a colori e un punteggio endoscopico viene applicato in base ai segienti parametri: <\/span><span style=\"font-weight: 400;\">\u00a0traslucenza del colon (0\u20133 punti), granulosit\u00e0 della mucosa (0\u20133 punti), morfologia del pattern vascolare (0\u20134 punti) e presenza di fibrina (0\u20134 punti). <\/span><\/p><p><span style=\"font-weight: 400;\">Il punteggio cumulativo varia da 0 (assenza di segni di infiammazione) a 14 (segni endoscopici di infiammazione molto grave).(<\/span><span style=\"font-weight: 400;\">1)<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-16e543e elementor-widget elementor-widget-image\" data-id=\"16e543e\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"748\" height=\"427\" src=\"https:\/\/phoenixlab1.com\/wp-content\/uploads\/2025\/07\/Capture23.png\" class=\"attachment-large size-large wp-image-2424\" alt=\"\" srcset=\"https:\/\/phoenixlab1.com\/wp-content\/uploads\/2025\/07\/Capture23.png 748w, https:\/\/phoenixlab1.com\/wp-content\/uploads\/2025\/07\/Capture23-300x171.png 300w\" sizes=\"(max-width: 748px) 100vw, 748px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-fb36707 elementor-widget elementor-widget-text-editor\" data-id=\"fb36707\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-weight: 400;\">(1) <\/span><span style=\"font-weight: 400;\">D\u2019Alessio S et al. <\/span><i><span style=\"font-weight: 400;\">J Clin Invest.<\/span><\/i><span style=\"font-weight: 400;\">\u00a02014;<\/span><a style=\"color: #cfaf7a;\" href=\"http:\/\/www.jci.org\/124\/9\"><span style=\"font-weight: 400;\">124(9)<\/span><\/a><span style=\"font-weight: 400;\">:3863-3878.\u00a0<\/span><a style=\"color: #cfaf7a;\" href=\"https:\/\/doi.org\/10.1172\/JCI72189\"><span style=\"font-weight: 400;\">https:\/\/doi.org\/10.1172\/JCI72189<\/span><\/a><span style=\"font-weight: 400;\">.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-9cecba4 elementor-widget elementor-widget-text-editor\" data-id=\"9cecba4\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-weight: 400;\">3) Parametri istologici: <\/span><b>RACHMILEWITZ (<\/b><b>1)<\/b> <span style=\"font-weight: 400;\">e<\/span><b> DIELEMAN <\/b><b>(<\/b><b>2) score<\/b><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-5d6741c elementor-widget__width-initial elementor-widget elementor-widget-text-editor\" data-id=\"5d6741c\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-weight: 400;\">Eseguiamo regolarmente analisi istologiche sui tessuti di colon murino. Un patologo esperto, in cieco rispetto al trattamento, valuta l\u2019infiammazione e i processi di riparazione epiteliale per assegnare un punteggio istologico, secondo quanto descritto da Rachmilewitz\u00b9 o Dieleman\u00b2 et al. Le immagini rappresentative mostrano che nei topi sani (es. Healthy + Vehicle) gli strati tissutali (mucosa, muscularis mucosae e sottomucosa) risultano ben strutturati. Nei topi con colite severa indotta da DSS (es. DSS + Vehicle), la stratificazione degli strati non \u00e8 pi\u00f9 mantenuta: un massiccio infiltrato infiammatorio (cerchi rossi) occupa completamente sia la mucosa che la sottomucosa, e le cripte risultano gravemente danneggiate (cerchio giallo) nella maggior parte del colon distale. L\u2019epitelio risulta quasi completamente eroso, indicando la presenza di ulcerazioni..<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-a34e991 elementor-widget elementor-widget-image\" data-id=\"a34e991\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"770\" height=\"424\" src=\"https:\/\/phoenixlab1.com\/wp-content\/uploads\/2025\/07\/Capture24.png\" class=\"attachment-large size-large wp-image-2431\" alt=\"\" srcset=\"https:\/\/phoenixlab1.com\/wp-content\/uploads\/2025\/07\/Capture24.png 770w, https:\/\/phoenixlab1.com\/wp-content\/uploads\/2025\/07\/Capture24-300x165.png 300w, https:\/\/phoenixlab1.com\/wp-content\/uploads\/2025\/07\/Capture24-768x423.png 768w\" sizes=\"(max-width: 770px) 100vw, 770px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-a1a384d elementor-widget elementor-widget-text-editor\" data-id=\"a1a384d\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-weight: 400;\">(1) <\/span><span style=\"font-weight: 400;\">Rachmilewitz, D. et al. <\/span><i><span style=\"font-weight: 400;\">Gastroenterology<\/span><\/i><span style=\"font-weight: 400;\"> 2002; 122: 1428\u20131441; <\/span><br \/><span style=\"font-weight: 400;\">(<\/span><span style=\"font-weight: 400;\">2)<\/span><span style=\"font-weight: 400;\">Dieleman, Palmen, Akol, et al. <\/span><i><span style=\"font-weight: 400;\">Clin Exp Immunol <\/span><\/i><span style=\"font-weight: 400;\">1998; 114: 385\u2013391.<\/span><br \/><span style=\"font-weight: 400;\">M: Mucosa; MM: Musculati mucosae; SM: Submucosa.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-c37e124 e-flex e-con-boxed e-con e-parent\" data-id=\"c37e124\" data-element_type=\"container\" data-e-type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-cfc8dd8 elementor-widget elementor-widget-text-editor\" data-id=\"cfc8dd8\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-weight: 400;\">4) <\/span><b>ANALISI IMMUNOISTOCHIMICHE<\/b><span style=\"font-weight: 400;\">. Eseguiamo di routine analisi immunoistochimiche su qualunque tipo di tessuto murino, incluso quello intestinale.\u00a0<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-009c22d elementor-widget elementor-widget-image\" data-id=\"009c22d\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"1013\" height=\"500\" src=\"https:\/\/phoenixlab1.com\/wp-content\/uploads\/2025\/07\/Capture25.png\" class=\"attachment-large size-large wp-image-2435\" alt=\"\" srcset=\"https:\/\/phoenixlab1.com\/wp-content\/uploads\/2025\/07\/Capture25.png 1013w, https:\/\/phoenixlab1.com\/wp-content\/uploads\/2025\/07\/Capture25-300x148.png 300w, https:\/\/phoenixlab1.com\/wp-content\/uploads\/2025\/07\/Capture25-768x379.png 768w\" sizes=\"(max-width: 1013px) 100vw, 1013px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-0e0bce2 elementor-widget elementor-widget-text-editor\" data-id=\"0e0bce2\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-weight: 400;\">5) <\/span><b>ANALISI AGGIUNTIVE<\/b><b>: <\/b><span style=\"font-weight: 400;\">FACS, RNAsequencing, Metagenomica, Lipidomica, Aanalisi bioinformatiche integrate, Multiplex, RT-PCR, KIT ELISA per citochine e chemochine<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-583f9e7 elementor-align-center elementor-widget elementor-widget-button\" data-id=\"583f9e7\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-button-wrapper\">\n\t\t\t\t\t<a class=\"elementor-button elementor-button-link elementor-size-sm\" href=\"https:\/\/phoenixlab1.com\/index.php\/presenta-proposta\/\">\n\t\t\t\t\t\t<span class=\"elementor-button-content-wrapper\">\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-button-text\">SCOPRI DI PIU'<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-1bd6be6 elementor-widget elementor-widget-spacer\" data-id=\"1bd6be6\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Modello acuto e cronico indotto dal Dextran sulfate sodium (DSS) \ud83d\udd0d\u00a0 PERCH\u00c9 \u00c8 UTILE? Il destrano solfato di sodio (DSS) [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"site-sidebar-layout":"no-sidebar","site-content-layout":"","ast-site-content-layout":"full-width-container","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"disabled","ast-breadcrumbs-content":"","ast-featured-img":"disabled","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"class_list":["post-2183","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/phoenixlab1.com\/index.php\/wp-json\/wp\/v2\/pages\/2183","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/phoenixlab1.com\/index.php\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/phoenixlab1.com\/index.php\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/phoenixlab1.com\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/phoenixlab1.com\/index.php\/wp-json\/wp\/v2\/comments?post=2183"}],"version-history":[{"count":58,"href":"https:\/\/phoenixlab1.com\/index.php\/wp-json\/wp\/v2\/pages\/2183\/revisions"}],"predecessor-version":[{"id":2762,"href":"https:\/\/phoenixlab1.com\/index.php\/wp-json\/wp\/v2\/pages\/2183\/revisions\/2762"}],"wp:attachment":[{"href":"https:\/\/phoenixlab1.com\/index.php\/wp-json\/wp\/v2\/media?parent=2183"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}