{"id":2216,"date":"2025-07-10T13:43:36","date_gmt":"2025-07-10T13:43:36","guid":{"rendered":"https:\/\/phoenixlab1.com\/?page_id=2216"},"modified":"2025-07-27T09:52:30","modified_gmt":"2025-07-27T09:52:30","slug":"inizio-espansione-e-crioconservazione-di-organoidi","status":"publish","type":"page","link":"https:\/\/phoenixlab1.com\/index.php\/inizio-espansione-e-crioconservazione-di-organoidi\/","title":{"rendered":"Inizio, espansione e crioconservazione di organoidi"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-page\" data-elementor-id=\"2216\" class=\"elementor elementor-2216\" data-elementor-settings=\"{&quot;element_pack_global_tooltip_width&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;element_pack_global_tooltip_width_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;element_pack_global_tooltip_width_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;element_pack_global_tooltip_padding&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true},&quot;element_pack_global_tooltip_padding_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true},&quot;element_pack_global_tooltip_padding_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true},&quot;element_pack_global_tooltip_border_radius&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true},&quot;element_pack_global_tooltip_border_radius_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true},&quot;element_pack_global_tooltip_border_radius_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true}}\">\n\t\t\t\t<div class=\"elementor-element elementor-element-eb862a6 e-con-full e-flex e-con e-parent\" data-id=\"eb862a6\" data-element_type=\"container\" data-e-type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;video&quot;,&quot;background_video_link&quot;:&quot;https:\\\/\\\/phoenixlab1.com\\\/wp-content\\\/uploads\\\/2025\\\/06\\\/Animation.mp4&quot;,&quot;background_video_start&quot;:0,&quot;background_play_on_mobile&quot;:&quot;yes&quot;}\">\n\t\t<div class=\"elementor-background-video-container\">\n\t\t\t\t\t\t\t<video class=\"elementor-background-video-hosted\" role=\"presentation\" autoplay muted playsinline loop><\/video>\n\t\t\t\t\t<\/div><div class=\"elementor-element elementor-element-b370696 e-flex e-con-boxed e-con e-child\" data-id=\"b370696\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-f4ce0d5 elementor-widget elementor-widget-heading\" data-id=\"f4ce0d5\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">INIZIO, ESPANSIONE E CRIOCONSERVAZIONE DI ORGANOIDI<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-c32ff63 e-flex e-con-boxed e-con e-parent\" data-id=\"c32ff63\" data-element_type=\"container\" data-e-type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-ba69f47 elementor-widget elementor-widget-spacer\" data-id=\"ba69f47\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-7434cf3 elementor-widget elementor-widget-heading\" data-id=\"7434cf3\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">INIZIO, ESPANSIONE E CRIOCONSERVAZIONE DI ORGANOIDI DERIVATI DA TESSUTO UMANO NORMALE E DA PAZIENTI CON IBD***, COLTIVATI SU MATRICE 3D<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-f737068 elementor-widget elementor-widget-text-editor\" data-id=\"f737068\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-weight: 400;\">Isoliamo ed espandiamo organoidi intestinali tridimensionali (3D) da biopsie endoscopiche o chirurgiche di pazienti con IBD o cancro del colon-retto, in seguito alla firma del consenso informato. Gli organoidi intestinali, che possono essere coltivati <\/span><i><span style=\"font-weight: 400;\">in vitro<\/span><\/i><span style=\"font-weight: 400;\">, presentano una struttura molto simile a quella del tessuto e dell\u2019organo di origine. Possono mimare le caratteristiche dei veri organi intestinali, inclusi morfologia, funzione e risposta personalizzata a stimoli specifici.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-928e7c2 elementor-widget elementor-widget-image\" data-id=\"928e7c2\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"1024\" height=\"379\" src=\"https:\/\/phoenixlab1.com\/wp-content\/uploads\/2025\/07\/Capture28-1024x379.png\" class=\"attachment-large size-large wp-image-2456\" alt=\"\" srcset=\"https:\/\/phoenixlab1.com\/wp-content\/uploads\/2025\/07\/Capture28-1024x379.png 1024w, https:\/\/phoenixlab1.com\/wp-content\/uploads\/2025\/07\/Capture28-300x111.png 300w, https:\/\/phoenixlab1.com\/wp-content\/uploads\/2025\/07\/Capture28-768x285.png 768w, https:\/\/phoenixlab1.com\/wp-content\/uploads\/2025\/07\/Capture28.png 1182w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-b0b8d3c elementor-widget elementor-widget-spacer\" data-id=\"b0b8d3c\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-fee2413 elementor-widget elementor-widget-heading\" data-id=\"fee2413\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Parametri di valutazione per l\u2019 efficacia terapeutica<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-d138f97 elementor-widget elementor-widget-text-editor\" data-id=\"d138f97\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Gli organoidi intestinali tridimensionali costituiscono un modello preclinico altamente affidabile per studi proof-of-concept e studi pilota. Possono essere sottoposti a trattamento con mezzi di coltura complessi contenenti piccole molecole, proteine ricombinanti, anticorpi e altri supplementi specifici per tessuto e patologia.<\/p><p><span style=\"font-weight: 400;\">Questi sistemi <\/span><i><span style=\"font-weight: 400;\">in vitro <\/span><\/i><span style=\"font-weight: 400;\">sono compatibili con un\u2019ampia gamma di analisi standard, tra cui l\u2019isolamento di RNA e DNA, l\u2019immunoistochimica e le tecniche di manipolazione genetica, consentendo un\u2019approfondita caratterizzazione molecolare e funzionale.<\/span><\/p><p><span style=\"font-weight: 400;\">Per valutare l\u2019 efficacia di un trattamento, proponiamo la valutazione dei seguenti parametri<\/span><span style=\"font-weight: 400;\"><br \/><\/span><\/p><ul><li aria-level=\"1\"><b>Proliferazione cellulare<\/b><\/li><li aria-level=\"1\"><b>Permeabilit\u00e0 della barriera epiteliale <\/b><span style=\"font-weight: 400;\">tramite resistenza elettrica transepiteliale (TEER)<\/span><\/li><li aria-level=\"1\"><b>Adesione batterica<\/b><\/li><li aria-level=\"1\"><span style=\"font-weight: 400;\">Valutazione delle <\/span><b>tight junctions <\/b><span style=\"font-weight: 400;\">e dei marcatori di differenziamento epiteliale<\/span><\/li><li aria-level=\"1\"><b>Transmigrazione di cellule immunitarie <\/b><span style=\"font-weight: 400;\">isolate dal sangue periferico (PBMC)<\/span><\/li><li aria-level=\"1\"><b>RNAsequencing<\/b><\/li><\/ul><ul><li style=\"list-style-type: none;\">\u00a0<\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-bc73266 elementor-align-center elementor-widget elementor-widget-button\" data-id=\"bc73266\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-button-wrapper\">\n\t\t\t\t\t<a class=\"elementor-button elementor-button-link elementor-size-sm\" href=\"https:\/\/phoenixlab1.com\/index.php\/presenta-proposta\/\">\n\t\t\t\t\t\t<span class=\"elementor-button-content-wrapper\">\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-button-text\">SCOPRI DI PIU'<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-e80a431 elementor-widget elementor-widget-spacer\" data-id=\"e80a431\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-a4c2cbe elementor-widget elementor-widget-text-editor\" data-id=\"a4c2cbe\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-weight: 400;\">***NOTA: <\/span><b>Tutti gli esperimenti condotti su campioni umani vengono eseguiti in conformit\u00e0 con le linee guida di Buona Pratica Clinica (GCP) e nel rispetto di tutte le normative e leggi internazionali vigenti, nonch\u00e9 di eventuali linee guida applicabili. Ogni protocollo, insieme al Consenso Informato firmato dai pazienti, viene sottoposto all\u2019approvazione del Comitato Etico competente.<\/b><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>INIZIO, ESPANSIONE E CRIOCONSERVAZIONE DI ORGANOIDI INIZIO, ESPANSIONE E CRIOCONSERVAZIONE DI ORGANOIDI DERIVATI DA TESSUTO UMANO NORMALE E DA PAZIENTI [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"site-sidebar-layout":"no-sidebar","site-content-layout":"","ast-site-content-layout":"full-width-container","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"disabled","ast-breadcrumbs-content":"","ast-featured-img":"disabled","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"class_list":["post-2216","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/phoenixlab1.com\/index.php\/wp-json\/wp\/v2\/pages\/2216","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/phoenixlab1.com\/index.php\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/phoenixlab1.com\/index.php\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/phoenixlab1.com\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/phoenixlab1.com\/index.php\/wp-json\/wp\/v2\/comments?post=2216"}],"version-history":[{"count":38,"href":"https:\/\/phoenixlab1.com\/index.php\/wp-json\/wp\/v2\/pages\/2216\/revisions"}],"predecessor-version":[{"id":2768,"href":"https:\/\/phoenixlab1.com\/index.php\/wp-json\/wp\/v2\/pages\/2216\/revisions\/2768"}],"wp:attachment":[{"href":"https:\/\/phoenixlab1.com\/index.php\/wp-json\/wp\/v2\/media?parent=2216"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}