{"id":2851,"date":"2025-07-27T11:23:44","date_gmt":"2025-07-27T11:23:44","guid":{"rendered":"https:\/\/phoenixlab1.com\/?page_id=2851"},"modified":"2025-07-27T17:59:14","modified_gmt":"2025-07-27T17:59:14","slug":"initiation-expansion-and-cryopreservation-of-organoids","status":"publish","type":"page","link":"https:\/\/phoenixlab1.com\/index.php\/en\/initiation-expansion-and-cryopreservation-of-organoids\/","title":{"rendered":"Initiation, expansion, and cryopreservation of organoids"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-page\" data-elementor-id=\"2851\" class=\"elementor elementor-2851\" data-elementor-settings=\"{&quot;element_pack_global_tooltip_width&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;element_pack_global_tooltip_width_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;element_pack_global_tooltip_width_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;element_pack_global_tooltip_padding&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true},&quot;element_pack_global_tooltip_padding_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true},&quot;element_pack_global_tooltip_padding_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true},&quot;element_pack_global_tooltip_border_radius&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true},&quot;element_pack_global_tooltip_border_radius_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true},&quot;element_pack_global_tooltip_border_radius_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true}}\">\n\t\t\t\t<div class=\"elementor-element elementor-element-eb862a6 e-con-full e-flex e-con e-parent\" data-id=\"eb862a6\" data-element_type=\"container\" data-e-type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;video&quot;,&quot;background_video_link&quot;:&quot;https:\\\/\\\/phoenixlab1.com\\\/wp-content\\\/uploads\\\/2025\\\/06\\\/Animation.mp4&quot;,&quot;background_video_start&quot;:0,&quot;background_play_on_mobile&quot;:&quot;yes&quot;}\">\n\t\t<div class=\"elementor-background-video-container\">\n\t\t\t\t\t\t\t<video class=\"elementor-background-video-hosted\" role=\"presentation\" autoplay muted playsinline loop><\/video>\n\t\t\t\t\t<\/div><div class=\"elementor-element elementor-element-b370696 e-flex e-con-boxed e-con e-child\" data-id=\"b370696\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-f4ce0d5 elementor-widget elementor-widget-heading\" data-id=\"f4ce0d5\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Initiation, expansion, and cryopreservation of organoids<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-c32ff63 e-flex e-con-boxed e-con e-parent\" data-id=\"c32ff63\" data-element_type=\"container\" data-e-type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-ba69f47 elementor-widget elementor-widget-spacer\" data-id=\"ba69f47\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-7434cf3 elementor-widget elementor-widget-heading\" data-id=\"7434cf3\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">INITIATION, EXPANSION, AND CRYOPRESERVATION OF ORGANOIDS DERIVED FROM NORMAL HUMAN TISSUE AND FROM PATIENTS WITH IBD***, CULTURED ON A 3D MATRIX<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-f737068 elementor-widget elementor-widget-text-editor\" data-id=\"f737068\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<span style=\"font-weight: 400;\">We isolate and expand three-dimensional (3D) intestinal organoids from endoscopic or surgical biopsies of patients with IBD or colorectal cancer, following the signing of informed consent. Intestinal organoids, which can be cultured <\/span><i><span style=\"font-weight: 400;\">in vitro<\/span><\/i><span style=\"font-weight: 400;\">, closely resemble the structure of the original tissue and organ. They can mimic the features of real intestinal organs, including morphology, function, and personalized responses to specific stimuli.<\/span>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-928e7c2 elementor-widget elementor-widget-image\" data-id=\"928e7c2\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"1024\" height=\"379\" src=\"https:\/\/phoenixlab1.com\/wp-content\/uploads\/2025\/07\/Capture28-1024x379.png\" class=\"attachment-large size-large wp-image-2456\" alt=\"\" srcset=\"https:\/\/phoenixlab1.com\/wp-content\/uploads\/2025\/07\/Capture28-1024x379.png 1024w, https:\/\/phoenixlab1.com\/wp-content\/uploads\/2025\/07\/Capture28-300x111.png 300w, https:\/\/phoenixlab1.com\/wp-content\/uploads\/2025\/07\/Capture28-768x285.png 768w, https:\/\/phoenixlab1.com\/wp-content\/uploads\/2025\/07\/Capture28.png 1182w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-b0b8d3c elementor-widget elementor-widget-spacer\" data-id=\"b0b8d3c\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-fee2413 elementor-widget elementor-widget-heading\" data-id=\"fee2413\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Evaluation parameters for therapeutic efficacy<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-d138f97 elementor-widget elementor-widget-text-editor\" data-id=\"d138f97\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Three-dimensional intestinal organoids represent a highly reliable preclinical model for proof-of-concept and pilot studies. They can be treated with complex culture media containing small molecules, recombinant proteins, antibodies, and other tissue- and disease-specific supplements.<\/p><p><span style=\"font-weight: 400;\">These <\/span><i><span style=\"font-weight: 400;\">in vitro<\/span><\/i><span style=\"font-weight: 400;\"> systems are compatible with a wide range of standard analyses, including RNA and DNA isolation, immunohistochemistry, and genetic manipulation techniques, enabling in-depth molecular and functional characterization.<\/span><\/p><p><span style=\"font-weight: 400;\">To assess treatment efficacy, we offer the evaluation of the following parameters:<\/span><\/p><ul><li aria-level=\"1\"><b>Cell proliferation<\/b><\/li><li aria-level=\"1\"><b>Permeability of the epithelial barrier<\/b><span style=\"font-weight: 400;\"> using transepithelial electrical resistance (TEER)<\/span><\/li><li aria-level=\"1\"><b>Bacterial adhesion<\/b><\/li><li aria-level=\"1\"><span style=\"font-weight: 400;\">Assessment of <\/span><b>tight junctions<\/b><span style=\"font-weight: 400;\"> and epithelial differentiation markers<\/span><\/li><li aria-level=\"1\"><b>Transmigration of immune cells<\/b><span style=\"font-weight: 400;\"> isolated from peripheral blood (PBMCs)<\/span><\/li><li aria-level=\"1\"><b>RNA sequencing<\/b><\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-bc73266 elementor-align-center elementor-widget elementor-widget-button\" data-id=\"bc73266\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-button-wrapper\">\n\t\t\t\t\t<a class=\"elementor-button elementor-button-link elementor-size-sm\" href=\"https:\/\/phoenixlab1.com\/index.php\/en\/request-proposal\/\">\n\t\t\t\t\t\t<span class=\"elementor-button-content-wrapper\">\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-button-text\">EXPLORE FURTHER<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-e80a431 elementor-widget elementor-widget-spacer\" data-id=\"e80a431\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-a4c2cbe elementor-widget elementor-widget-text-editor\" data-id=\"a4c2cbe\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<span style=\"font-weight: 400;\">***NOTE: <\/span><b>All experiments conducted on human samples are carried out in accordance with Good Clinical Practice (GCP) guidelines and in compliance with all applicable international laws and regulations, as well as any relevant guidelines. Each protocol, along with the Informed Consent signed by patients, is submitted for approval to the appropriate Ethics Committee.<\/b>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Initiation, expansion, and cryopreservation of organoids INITIATION, EXPANSION, AND CRYOPRESERVATION OF ORGANOIDS DERIVED FROM NORMAL HUMAN TISSUE AND FROM PATIENTS [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"site-sidebar-layout":"no-sidebar","site-content-layout":"","ast-site-content-layout":"full-width-container","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"disabled","ast-breadcrumbs-content":"","ast-featured-img":"disabled","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"class_list":["post-2851","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/phoenixlab1.com\/index.php\/wp-json\/wp\/v2\/pages\/2851","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/phoenixlab1.com\/index.php\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/phoenixlab1.com\/index.php\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/phoenixlab1.com\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/phoenixlab1.com\/index.php\/wp-json\/wp\/v2\/comments?post=2851"}],"version-history":[{"count":7,"href":"https:\/\/phoenixlab1.com\/index.php\/wp-json\/wp\/v2\/pages\/2851\/revisions"}],"predecessor-version":[{"id":2905,"href":"https:\/\/phoenixlab1.com\/index.php\/wp-json\/wp\/v2\/pages\/2851\/revisions\/2905"}],"wp:attachment":[{"href":"https:\/\/phoenixlab1.com\/index.php\/wp-json\/wp\/v2\/media?parent=2851"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}