{"id":2858,"date":"2025-07-27T17:45:33","date_gmt":"2025-07-27T17:45:33","guid":{"rendered":"https:\/\/phoenixlab1.com\/?page_id=2858"},"modified":"2025-07-30T15:03:17","modified_gmt":"2025-07-30T15:03:17","slug":"ex-vivo-cultures-of-intestinal-biopsies-from-patients","status":"publish","type":"page","link":"https:\/\/phoenixlab1.com\/index.php\/en\/ex-vivo-cultures-of-intestinal-biopsies-from-patients\/","title":{"rendered":"Ex vivo cultures of intestinal biopsies from patients"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-page\" data-elementor-id=\"2858\" class=\"elementor elementor-2858\" data-elementor-settings=\"{&quot;element_pack_global_tooltip_width&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;element_pack_global_tooltip_width_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;element_pack_global_tooltip_width_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;element_pack_global_tooltip_padding&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true},&quot;element_pack_global_tooltip_padding_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true},&quot;element_pack_global_tooltip_padding_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true},&quot;element_pack_global_tooltip_border_radius&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true},&quot;element_pack_global_tooltip_border_radius_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true},&quot;element_pack_global_tooltip_border_radius_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true}}\">\n\t\t\t\t<div class=\"elementor-element elementor-element-c20fb64 e-con-full e-flex e-con e-parent\" data-id=\"c20fb64\" data-element_type=\"container\" data-e-type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;video&quot;,&quot;background_video_link&quot;:&quot;https:\\\/\\\/phoenixlab1.com\\\/wp-content\\\/uploads\\\/2025\\\/06\\\/Animation.mp4&quot;,&quot;background_video_start&quot;:0,&quot;background_play_on_mobile&quot;:&quot;yes&quot;}\">\n\t\t<div class=\"elementor-background-video-container\">\n\t\t\t\t\t\t\t<video class=\"elementor-background-video-hosted\" role=\"presentation\" autoplay muted playsinline loop><\/video>\n\t\t\t\t\t<\/div><div class=\"elementor-element elementor-element-1f97416 e-flex e-con-boxed e-con e-child\" data-id=\"1f97416\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-3b60a1c elementor-widget elementor-widget-heading\" data-id=\"3b60a1c\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\"><i>EX VIVO<\/i> CULTURES OF INTESTINAL BIOPSIES FROM PATIENTS WITH IBD<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-7f34346 e-flex e-con-boxed e-con e-parent\" data-id=\"7f34346\" data-element_type=\"container\" data-e-type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-449f078 elementor-widget elementor-widget-spacer\" data-id=\"449f078\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-199b462 elementor-widget elementor-widget-heading\" data-id=\"199b462\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">EX VIVO CULTURES OF INTESTINAL BIOPSIES FROM PATIENTS WITH IBD ***\n<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ee74a91 elementor-widget elementor-widget-text-editor\" data-id=\"ee74a91\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<span style=\"font-weight: 400;\">Ex vivo<\/span><i><span style=\"font-weight: 400;\"> intestinal biopsy cultures<\/span><\/i><span style=\"font-weight: 400;\"> from patients with IBD represent an advanced platform for preclinical drug testing, as they preserve the <\/span><i><span style=\"font-weight: 400;\">in vivo<\/span><\/i><span style=\"font-weight: 400;\"> tissue architecture and faithfully reflect the pathological alterations of these diseases.<\/span>\n\n<span style=\"font-weight: 400;\">This methodology allows for the study of cells within their native microenvironment, maintaining the integrity of both cellular and extracellular structures for up to 24 hours after sample collection.<\/span>\n\n<span style=\"font-weight: 400;\">Tissue responses to stimuli reflect not only the activity of the drug but also cell\u2013matrix interactions, providing results that are highly clinically relevant.<\/span>\n\n<span style=\"font-weight: 400;\">We use endoscopic biopsies from patients with IBD or colorectal cancer, as well as from healthy donors, following the signing of informed consent and approval by the competent Ethics Committee, to generate personalized intestinal biopsy cultures.<\/span>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-bf43d69 elementor-widget__width-initial elementor-widget elementor-widget-image\" data-id=\"bf43d69\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"1024\" height=\"452\" src=\"https:\/\/phoenixlab1.com\/wp-content\/uploads\/2025\/07\/Immagine21-1024x452.jpg\" class=\"attachment-large size-large wp-image-2990\" alt=\"\" srcset=\"https:\/\/phoenixlab1.com\/wp-content\/uploads\/2025\/07\/Immagine21-1024x452.jpg 1024w, https:\/\/phoenixlab1.com\/wp-content\/uploads\/2025\/07\/Immagine21-300x132.jpg 300w, https:\/\/phoenixlab1.com\/wp-content\/uploads\/2025\/07\/Immagine21-768x339.jpg 768w, https:\/\/phoenixlab1.com\/wp-content\/uploads\/2025\/07\/Immagine21-1536x678.jpg 1536w, https:\/\/phoenixlab1.com\/wp-content\/uploads\/2025\/07\/Immagine21-2048x904.jpg 2048w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-006f0aa elementor-widget elementor-widget-text-editor\" data-id=\"006f0aa\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-weight: 400;\">To assess the efficacy of a treatment, we offer the evaluation of the following parameters:<\/span><br \/><b><\/b><\/p><ul><li><b>RNA sequencing<\/b><span style=\"font-weight: 400;\"> on tissue biopsies<\/span><\/li><li><b style=\"font-style: inherit;\">Luminex Discovery 35-plex<\/b><span style=\"font-weight: 400;\"> on supernatants for quantification of inflammatory cytokines\/chemokines<\/span><\/li><li><b style=\"font-style: inherit;\">Immunohistochemistry<\/b><span style=\"font-weight: 400;\"> on tissue biopsies<\/span><\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-823700e elementor-widget elementor-widget-spacer\" data-id=\"823700e\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-86411d2 elementor-align-center elementor-widget elementor-widget-button\" data-id=\"86411d2\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-button-wrapper\">\n\t\t\t\t\t<a class=\"elementor-button elementor-button-link elementor-size-sm\" href=\"https:\/\/phoenixlab1.com\/index.php\/en\/request-proposal\/\">\n\t\t\t\t\t\t<span class=\"elementor-button-content-wrapper\">\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-button-text\">EXPLORE FURTHER<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-2aeb7c8 elementor-widget elementor-widget-spacer\" data-id=\"2aeb7c8\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-d387a6e elementor-widget elementor-widget-text-editor\" data-id=\"d387a6e\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<span style=\"font-weight: 400;\">***NOTE: <\/span><b>All experiments conducted on human samples are carried out in accordance with Good Clinical Practice (GCP) guidelines and in compliance with all applicable international laws and regulations, as well as any relevant guidelines. Each protocol, along with the Informed Consent signed by patients, is submitted for approval to the appropriate Ethics Committee.<\/b>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>EX VIVO CULTURES OF INTESTINAL BIOPSIES FROM PATIENTS WITH IBD EX VIVO CULTURES OF INTESTINAL BIOPSIES FROM PATIENTS WITH IBD [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"site-sidebar-layout":"no-sidebar","site-content-layout":"","ast-site-content-layout":"full-width-container","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"disabled","ast-breadcrumbs-content":"","ast-featured-img":"disabled","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"class_list":["post-2858","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/phoenixlab1.com\/index.php\/wp-json\/wp\/v2\/pages\/2858","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/phoenixlab1.com\/index.php\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/phoenixlab1.com\/index.php\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/phoenixlab1.com\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/phoenixlab1.com\/index.php\/wp-json\/wp\/v2\/comments?post=2858"}],"version-history":[{"count":13,"href":"https:\/\/phoenixlab1.com\/index.php\/wp-json\/wp\/v2\/pages\/2858\/revisions"}],"predecessor-version":[{"id":2996,"href":"https:\/\/phoenixlab1.com\/index.php\/wp-json\/wp\/v2\/pages\/2858\/revisions\/2996"}],"wp:attachment":[{"href":"https:\/\/phoenixlab1.com\/index.php\/wp-json\/wp\/v2\/media?parent=2858"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}