Initiation, expansion, and cryopreservation of organoids
INITIATION, EXPANSION, AND CRYOPRESERVATION OF ORGANOIDS DERIVED FROM NORMAL HUMAN TISSUE AND FROM PATIENTS WITH IBD***, CULTURED ON A 3D MATRIX
We isolate and expand three-dimensional (3D) intestinal organoids from endoscopic or surgical biopsies of patients with IBD or colorectal cancer, following the signing of informed consent. Intestinal organoids, which can be cultured in vitro, closely resemble the structure of the original tissue and organ. They can mimic the features of real intestinal organs, including morphology, function, and personalized responses to specific stimuli.
Evaluation parameters for therapeutic efficacy
Three-dimensional intestinal organoids represent a highly reliable preclinical model for proof-of-concept and pilot studies. They can be treated with complex culture media containing small molecules, recombinant proteins, antibodies, and other tissue- and disease-specific supplements.
These in vitro systems are compatible with a wide range of standard analyses, including RNA and DNA isolation, immunohistochemistry, and genetic manipulation techniques, enabling in-depth molecular and functional characterization.
To assess treatment efficacy, we offer the evaluation of the following parameters:
- Cell proliferation
- Permeability of the epithelial barrier using transepithelial electrical resistance (TEER)
- Bacterial adhesion
- Assessment of tight junctions and epithelial differentiation markers
- Transmigration of immune cells isolated from peripheral blood (PBMCs)
- RNA sequencing
***NOTE: All experiments conducted on human samples are carried out in accordance with Good Clinical Practice (GCP) guidelines and in compliance with all applicable international laws and regulations, as well as any relevant guidelines. Each protocol, along with the Informed Consent signed by patients, is submitted for approval to the appropriate Ethics Committee.